An injected simulation system of infrared detectors based on Wishbone-PCI bridge core and Sprtan6 series FPGA were introduced. The design and implementation of the injected interface card of infrared image based on PCI bus and technology of Wishbone-PCI bridge core was discussed in detail. Replacing the head detector, the system can inject the simulation image into the real-time processing tank of infrared warning and tracking system, which can verify and simulate the target detection and tracking function. The system superposes infrared image data collected by infrared detection warning system and the custom target image, injecting to the real-time processing tank, so the system can present real and continuous data and can simulate realistic scenarios. It can provide true and reliable data source for real-time signal processing tank. It can speed up the development process of infrared detection and warning systems and shorten the development period.